asnH [2017-8-11 16:0:3]

asparagine synthase (glutamine-hydrolysing)

locus: BSU39920

pI: 5.53

mw: 85.66 kDa

protein length: 747 aa

gene length: 2241 bp

function: biosynthesis of asparagine

product: asparagine synthase (glutamine-hydrolysing))

essential: no

ec: 6.3.5.4

synonyms: yxaN

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.5|Biosynthesis/ acquisition of aspartate/ asparagine]

Gene

Coordinates: 4,098,926 → 4,101,169

The protein

Catalyzed reaction/ biological activity

• ATP L-aspartate L-glutamine H₂O = AMP diphosphate L-asparagine L-glutamate (according to Swiss-Prot)

Protein family

• asparagine synthetase family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|787BB72119DB1321A8281D7C46FED28CAEDBC97C|yxbB]-[gene|88B01CC87394AEE693A98574F9343EBF99F0D60E|yxbA]-[gene|A15368CD02487EBC1AF999FED34672E38167BCB2|yxnB]-[gene|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH]-[gene|BF0E188249161B3CC24D223FDD48B820EBED8F55|yxaM]
  description: [Pubmed|10746760]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20185509], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|15101989], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed by glucose (8-fold) [Pubmed|12850135]

Biological materials

Mutant

• MGNA-B689 (asnH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1688 NBRP B. subtilis, Japan]

References

Reviews

• 12859215,11395405

Original publications

• 20185509,10746760,12618455,10498721,12618455,15101989